Study biological processes at molecular level

Molecular Biology screenshot
Molecular Biology screenshot
Molecular Biology screenshot
Molecular Biology screenshot
Molecular Biology screenshot
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Flashcard Decks
Curriculum

What You'll Learn

01 Introduction to Molecular Biology
3 topics
1 Historical Perspective
  • Discovery of DNA Structure
  • Central Dogma of Molecular Biology
  • Key Milestones in Molecular Biology
2 Molecular Biology and Other Disciplines
  • Relationship with Biochemistry
  • Relationship with Genetics
  • Relationship with Cell Biology
3 Methods in Molecular Biology
  • Overview of Techniques
  • Model Organisms
  • Ethical Considerations
02 Chemical Foundations of Molecular Biology
3 topics
1 Water and Non-covalent Interactions
  • Hydrogen Bonding
  • Hydrophobic Interactions
  • Van der Waals Forces
  • Ionic Interactions
2 Organic Chemistry Principles
  • Carbon Chemistry
  • Functional Groups
  • Isomerism
3 Biological Macromolecules
  • Properties of Macromolecules
  • Types of Biological Polymers
  • Macromolecular Assembly
03 Nucleic Acid Structure and Function
3 topics
1 Nucleotides
  • Chemical Structure of Nucleotides
  • Nucleoside vs. Nucleotide
  • Modified Nucleotides
2 DNA Structure
  • Primary Structure
  • Secondary Structure (B, A, and Z-DNA)
  • Tertiary Structure (DNA Packaging)
  • Denaturation and Renaturation
3 RNA Structure
  • Types of RNA
  • RNA Secondary Structures
  • RNA Tertiary Structures
  • Ribozymes and Catalytic RNA
04 Genome Organization
3 topics
1 Prokaryotic Genomes
  • Bacterial Chromosomes
  • Plasmids
  • Bacteriophage Genomes
2 Eukaryotic Genomes
  • Nuclear Genome Organization
  • Chromosomal Structure
  • Organellar Genomes (Mitochondria and Chloroplasts)
3 Genome Complexity
  • C-Value Paradox
  • Repetitive DNA
  • Unique Sequences
05 Chromatin Structure and Function
3 topics
1 Nucleosomes
  • Histone Proteins
  • Nucleosome Assembly
  • Higher-Order Structure
2 Chromatin Remodeling
  • ATP-Dependent Remodeling Complexes
  • Histone Variants
  • Histone Chaperones
3 Epigenetic Modifications
  • Histone Modifications
  • DNA Methylation
  • Non-coding RNAs in Chromatin Regulation
06 DNA Replication
5 topics
1 Basic Principles of DNA Replication
  • Semiconservative Replication
  • Bidirectional Replication
  • Origins of Replication
2 DNA Replication Machinery
  • DNA Polymerases
  • Helicases and Topoisomerases
  • Primase and Ligase
  • Single-Stranded Binding Proteins
3 Mechanics of Replication
  • Initiation
  • Elongation
  • Termination
  • Leading and Lagging Strand Synthesis
4 Differences in Replication
  • Prokaryotic vs. Eukaryotic Replication
  • Telomere Replication
  • Mitochondrial DNA Replication
5 Regulation of DNA Replication
  • Cell Cycle Control
  • Licensing of Replication Origins
  • Checkpoint Control
07 DNA Damage, Repair, and Recombination
4 topics
1 Types of DNA Damage
  • Spontaneous Lesions
  • Environmental Damage
  • Replication Errors
2 DNA Repair Mechanisms
  • Direct Repair
  • Base Excision Repair
  • Nucleotide Excision Repair
  • Mismatch Repair
  • Double-Strand Break Repair
3 Recombination
  • Homologous Recombination
  • Site-Specific Recombination
  • Transposition
4 DNA Damage Response
  • Damage Sensing
  • Signal Transduction
  • Checkpoints and Cell Cycle Arrest
  • Apoptosis
08 Transcription in Prokaryotes
3 topics
1 RNA Polymerase
  • Structure and Function
  • Sigma Factors
  • Core Enzyme vs. Holoenzyme
2 Transcription Process
  • Initiation
  • Elongation
  • Termination (Rho-Dependent and Independent)
3 Regulation of Transcription
  • Promoter Structure and Recognition
  • Operators and Repressors
  • Positive Regulation and Activators
  • Attenuation
  • Riboswitches
09 Transcription in Eukaryotes
3 topics
1 RNA Polymerases
  • RNA Polymerase I, II, and III
  • Structure and Function
  • General Transcription Factors
2 Transcription Process
  • Initiation and Pre-Initiation Complex
  • Elongation
  • Termination
3 Regulation of Transcription
  • Promoter Structure
  • Enhancers and Silencers
  • Transcription Factors and Coactivators
  • Mediator Complex
  • Chromatin-Based Regulation
10 RNA Processing
5 topics
1 5' Capping
  • Cap Structure
  • Capping Enzymes
  • Function of the Cap
2 3' Polyadenylation
  • Poly(A) Signal Recognition
  • Cleavage and Polyadenylation
  • Function of the Poly(A) Tail
3 RNA Splicing
  • Introns and Exons
  • Splicing Mechanism
  • Spliceosome Assembly and Function
  • Alternative Splicing
  • Self-Splicing Introns
4 RNA Editing
  • Types of RNA Editing
  • Mechanism of Editing
  • Biological Significance
5 RNA Stability and Degradation
  • mRNA Half-Life
  • Degradation Pathways
  • Regulation of mRNA Stability
11 Translation
5 topics
1 The Genetic Code
  • Triplet Nature
  • Degeneracy and Wobble
  • Universal vs. Non-Universal Codes
2 Transfer RNA
  • Structure of tRNA
  • Aminoacylation of tRNA
  • Codon-Anticodon Recognition
3 Ribosomes
  • Structure and Composition
  • Functional Sites
  • Prokaryotic vs. Eukaryotic Ribosomes
4 Translation Process
  • Initiation
  • Elongation
  • Termination
  • Recycling
5 Regulation of Translation
  • Initiation Factors
  • Regulatory Elements in mRNA
  • microRNA-Mediated Regulation
  • RNA-Binding Proteins
12 Protein Structure and Function
4 topics
1 Amino Acids and Peptide Bonds
  • Properties of Amino Acids
  • Peptide Bond Formation
  • Polypeptide Chains
2 Protein Structure
  • Primary Structure
  • Secondary Structure (α-Helix, β-Sheet)
  • Tertiary Structure and Domains
  • Quaternary Structure
  • Protein Folding and Chaperones
3 Protein Function
  • Enzyme Catalysis
  • Structural Proteins
  • Transport Proteins
  • Signaling Proteins
4 Post-Translational Modifications
  • Types of Modifications
  • Enzymes Involved
  • Functional Consequences
13 Gene Regulation in Prokaryotes
4 topics
1 Operon Structure and Function
  • Lac Operon
  • Trp Operon
  • Other Operons
2 Negative Regulation
  • Repressor Proteins
  • Operator Sites
  • Corepressors
3 Positive Regulation
  • Activator Proteins
  • Catabolite Activation Protein (CAP)
  • Antitermination
4 Global Regulatory Systems
  • Stringent Response
  • Heat Shock Response
  • Two-Component Systems
14 Gene Regulation in Eukaryotes
4 topics
1 Transcriptional Regulation
  • Promoter Structure and Function
  • Enhancers and Silencers
  • Transcription Factor Networks
  • Chromatin Remodeling and Histone Modifications
2 Post-Transcriptional Regulation
  • Alternative Splicing
  • mRNA Stability and Degradation
  • RNA Interference
  • Non-Coding RNAs
3 Translational and Post-Translational Regulation
  • Translation Initiation Control
  • Protein Degradation and the Ubiquitin System
  • Protein Modification and Signaling
4 Epigenetic Regulation
  • DNA Methylation
  • Histone Code
  • Epigenetic Inheritance
15 Molecular Signaling
4 topics
1 Signal Transduction Pathways
  • Receptors and Ligands
  • Second Messengers
  • Protein Kinases and Phosphatases
2 Major Signaling Pathways
  • G-Protein Coupled Receptors
  • Receptor Tyrosine Kinases
  • JAK-STAT Pathway
  • TGF-β Pathway
  • Notch Signaling
3 Signal Integration and Cross-Talk
  • Feedback Loops
  • Scaffold Proteins
  • Network Motifs
4 Nuclear Receptor Signaling
  • Structure of Nuclear Receptors
  • Steroid Hormone Receptors
  • Non-Steroid Nuclear Receptors
16 Molecular Techniques
5 topics
1 Nucleic Acid Manipulation
  • Restriction Enzymes
  • Ligases and Polymerases
  • Cloning Vectors
  • Transformation and Transfection
2 DNA Amplification and Sequencing
  • Polymerase Chain Reaction (PCR)
  • Real-Time PCR
  • Traditional Sequencing Methods
  • Next-Generation Sequencing
3 Gene Expression Analysis
  • Northern Blotting
  • RT-PCR
  • Microarrays
  • RNA-Seq
4 Protein Analysis
  • Western Blotting
  • Immunoprecipitation
  • Mass Spectrometry
  • Proteomics
5 Genetic Engineering
  • Site-Directed Mutagenesis
  • Gene Knockouts and Knockdowns
  • CRISPR-Cas9 Technology
  • Synthetic Biology
17 Molecular Evolution
4 topics
1 Molecular Basis of Evolution
  • Mutations and Genetic Variation
  • Selection at the Molecular Level
  • Neutral Theory
2 Molecular Phylogenetics
  • Sequence Alignment
  • Phylogenetic Tree Construction
  • Molecular Clocks
3 Genome Evolution
  • Gene Duplication and Divergence
  • Horizontal Gene Transfer
  • Mobile Genetic Elements
  • Whole Genome Duplication
4 Molecular Coevolution
  • Host-Pathogen Interactions
  • Evolutionary Arms Races
  • Molecular Adaptation
18 Systems and Synthetic Molecular Biology
4 topics
1 Systems Biology Approaches
  • Network Models
  • High-Throughput Data Integration
  • Computational Modeling
2 'Omics Technologies
  • Genomics
  • Transcriptomics
  • Proteomics
  • Metabolomics
  • Interactomics
3 Synthetic Biology
  • Biological Parts and BioBricks
  • Genetic Circuits
  • Minimal Genomes
  • Expanded Genetic Code
4 Applied Molecular Biology
  • Molecular Diagnostics
  • Molecular Therapeutics
  • Biopharmaceuticals
  • Gene Therapy
19 Specialized Topics in Molecular Biology
5 topics
1 Developmental Molecular Biology
  • Gene Regulatory Networks in Development
  • Epigenetic Reprogramming
  • Morphogen Gradients
2 Cancer Molecular Biology
  • Oncogenes and Tumor Suppressors
  • DNA Repair Defects and Genomic Instability
  • Molecular Basis of Metastasis
  • Molecular Diagnostics and Targeted Therapies
3 Molecular Immunology
  • Antibody Diversity Generation
  • Major Histocompatibility Complex
  • Molecular Basis of Immune Recognition
4 Molecular Virology
  • Viral Genomes and Gene Expression
  • Virus-Host Interactions
  • Viral Evolution and Emerging Diseases
5 Molecular Neurobiology
  • Molecular Basis of Synaptic Transmission
  • Molecular Mechanisms of Learning and Memory
  • Neurogenetic Disorders
20 Current Research and Future Directions
4 topics
1 Emerging Technologies
  • Single-Cell Analysis
  • Spatial Transcriptomics
  • Genome Editing Advances
  • Long-Read Sequencing
2 Frontier Areas of Research
  • Epitranscriptomics
  • Phase Separation in Cell Biology
  • RNA-Based Therapeutics
  • Structural Genomics
3 Integrative Approaches
  • Multi-Omics Integration
  • Molecular Systems Medicine
  • Digital Biology and Artificial Intelligence
4 Ethical and Societal Implications
  • Gene Editing Ethics
  • Privacy and Genetic Information
  • Molecular Biology and Bioeconomy
  • Biosafety and Biosecurity

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